Cyclin D1 (L283) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1741-50, BS1741-100

Sizes: 50µl, 100µl

Swiss-Prot: P24385

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cyclin D1 (L283) polyclonal antibody detects endogenous levels of Cyclin D1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cyclin D1 (L283) pAb at 1:2000 dilution
Lane 1: HepG2 whole cell lysate (20ug)
Lane 2: L02 whole cell lysate (20ug)
Lane 3: C6 whole cell lysate (20ug)
Lane 4: 3T3-L1 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of Cyclin D1 (L283) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 36 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1

Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human Cyclin D1.

Conjugate: Unconjugated

Modification: Unmodified